Isolation, Characterization and Phylogenetic Analysis of Nodule-Associated Bacteria from Mimosa Pudica L.

The interaction between rhizobia and other nodule-associated bacteria assists to mitigate nutrient stress in leguminous plants by fixing atmospheric nitrogen synthesizing plant growth regulators. beneficial effects of microbial inoculants emphasize the need for further research their use modern agriculture. present study describes isolation, molecular identification, characterization, phylogenetic analysis noduleassociated from Mimosa pudica Linnaeus. Isolation phenotypic characterization were carried out according standard procedures. Molecular isolates was performed using 16S ribosomal RNA. Plant promoting ability selected analyzed assessing indole acetic acid production, nitrogenfixing organic production. Evolutionary distance relatedness neighbor-joining method. Thirteen isolated identified rRNA gene sequencing. such as Rhizobium sp. CU8 three co-resident non-rhizobial (Bacillus cereus MY5, Ralstonia pickettii MY1 Lactococcus lactis MY3) exhibited promotion potential activities. Phylogenetic revealed genetic evolutionary significance all thirteen reside root nodule M. pudica. four with properties. L. MY3 is first report a promoter nodules